transport processes and separation principles solutions manual

The output of the detector is a graph, called Chromatogram. Each component in the sample interacts slightly different with the adsorbent material, causing different migration rate for the each component, leading to their separation, as they flow out of the column into a specific detector. It relies on high pressure pumps, which deliver mixtures of various solvents, called the mobile phase, which flows through the system, collecting the sample mixture on the way, delivering it into a cylinder, called the column, filled with solid particles, made of adsorbent material, called the stationary phase. The mixtures can originated from food, chemicals, pharmaceuticals, biological, environmental and agriculture, etc, which have been dissolved into liquid solutions. High-performance liquid chromatography ( HPLC), formerly referred to as high-pressure liquid chromatography, is a technique in analytical chemistry used to separate, identify, and quantify specific components in mixtures. (1) Solvent reservoirs, (2) Solvent degasser, (3) Gradient valve, (4) Mixing vessel for delivery of the mobile phase, (5) High-pressure pump, (6) Switching valve in 'inject position', (6') Switching valve in 'load position', (7) Sample injection loop, (8) Pre-column (guard column), (9) Analytical column, (10) Detector ( i.e., IR, UV), (11) Data acquisition, (12) Waste or fraction collector. Schematic representation of an HPLC unit.

Transport processes and separation principles solutions manual